The impact of ovary hormone deficiency on high fat and high sugar diet - induced overactive bladder in a rat model

Juan Y1, Chuang S2, Lu J3, Long C4, Lin K5, Wu W6, Lee Y7, Chen S7

Research Type

Pure and Applied Science / Translational

Abstract Category

Overactive Bladder

Abstract 129
Open Discussion ePosters
Scientific Open Discussion Session 7
Wednesday 29th August 2018
12:20 - 12:25 (ePoster Station 7)
Exhibition Hall
Basic Science Molecular Biology Overactive Bladder
1. Department of Urology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan;, 2. Translational Research Center, Cancer Center, Department of Medical Research, Kaohsiung Medical University, Kaohsiung, Taiwan, 3. Graduate Institute of Medical Science, Kaohsiung Medical University, Kaohsiung, Taiwan, 4. Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, 5. Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;, 6. Department of Urology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan., 7. Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan
Presenter
C

Cheng-Yu Long

Links

Abstract

Hypothesis / aims of study
The pathophysiology mechanism of menopause in the metabolic syndrome associated bladder dysfunction is still not clear. The major aims of the present study were to examine the impact of high-fat-high-sugar diet and surgical menopause - induced metabolic syndrome in overactive bladder.
Study design, materials and methods
Female Sprague-Dawley rats were feed with high-fat-high-sugar diet with/without ovariectomy surgery to mimic menopause and to induce metabolic syndrome. At six months after high-fat-high-sugar feeding, cystometrogram, physical indicator, urine and serum biochemistry parameters were measured. Masson’s trichrome stain and Western blots were carried out to examine the expressions of interstitial fibrosis, fibrosis-associated proteins and muscarinic or purinergic receptors.
Results
Bladder hyperactivity was induced accompanied by bladder interstitial fibrosis after 6 months of high-fat-high-sugar feeding, while surgical menopause exacerbated these bladder damages and enhanced interstitial fibrosis level. In addition, surgical menopause enhanced bladder dysfunction via over-expression of muscarinic and purinergic receptors.
Interpretation of results
HFHS diet for 6 months clearly induced metabolic syndrome. These HFHS and OVX-HFHS treated rats also increased micturition frequency and peak micturition pressure, enhanced the expression of P2X3 purinergic receptors, and raised oxidative stress and interstitial fibrosis. Chronic inflammation and myopathy induced by metabolic perturbations were the causes of bladder malfunction.  Ovariectomy significantly increased the bladder fibrosis, enhanced bladder fibrosis markers, expanded bladder apoptotic cells and augmented oxidative stress damage
Concluding message
High-fat-high-sugar feeding induced bladder overactivity, while ovary hormone deficiency enhanced bladder interstitial fibrosis, exacerbated overactive bladder syndrome and increased muscarinic and purinergic receptors expressions.
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Disclosures
Funding None Clinical Trial No Subjects Animal Species Rat Ethics Committee Affidative of approval use protocol Kaohsiung Medical University
27/03/2024 18:04:43