To analyse the bio-psycho-sexual determinants that influence irritable bowel syndrome

It is a Non-experimental study with Correlation type, sampling method was convenient sampling,15 participants were recruited according to the prevalence rate regionally. women with age group of 25- 35 years, who were diagnosed to have a IBS using ROME III criteria were included, participants those who were not willing to participate, and with Known psychological diseases and carcinoma, Sexual dysfunction, menstrual disorders were excluded from the study.
PROCEDURE
This study was presented in Institutional ethical committee and got approved with the reference number :3072/IEC/2022 
Participants were selected based on the inclusion and exclusion criteria,Then the Informed consent was obtained,Self reported questionnaires distributed to the participants related to physical activity(IPAQ), Sexual function(FSFI) and Social Behavior (IBS-BRQ) and Stress (PSQ) ,Serum samples were collected from the participants, Hormone levels (Estrogen ,Progesterone and Cortisol) were analysed.
 
•	Following the aseptic protocol, approximately 5 ml of venous blood sample was drawn from the participants in plain vacutainer .After centrifugation, the serum sample were separated and stored in deep freezer at -20 degree C for further analysis. 
Serum samples were analysed using Competitive ELISA  washer and reader method.

•	Table shows the correlation between the factors influencing IBS

Factors 	Age 	PA	Stress	FSFI	S.
Proges
terone	S.
Cortical	S. Estrogen	Behaviour 
Age 	1	.233	-0.123	-0.318	0.337	-0.042	0.095	-0.060
PA	0.223	1	-0.0134	-0.008	-0.164	0.175	0.149	0.160
Stress 	-0.123	-0.134	1	0.319	-0.227	0.238	0.375	0.521
FSFI	-0.318	-0.008	0.319	1	-0.630	-0.022	0.080	0.069
S.
Progesterone	0.337	-0.164	-0.227	-0.630	1	-0.143	-0.168	-0.203
S.Cortical	-0.042	0.175	0.238	-0.022	-0.143	1	-0.032	0.217
S.Estrogen	0.095	0.149	0.357	0.080	-0.168	-0.032	1	0.227
Behaviour 	-0.060	0.160	0.521	0.069	-0.230	0.217	0.227	1

50% of study participants reported sexual dysfunction based on the FSFI scores.  The presence  of IBS symptoms  entails a significant somatic  stress that may  influence  the  sexual functions. Decreased libido  to be the  most common symptom perceived by most of the participants

Our findings conclude that there was a Highly positive correlation between social behavior and perceived stress .And also found a Positive correlation among Serum Cortisol and serum estrogen and FSFI Scores. Further more we have observed negative correlation between age, physical activity and Serum progesterone.
Study results were strongly supporting that perceived stress level and fluctuation of sex hormones are predominant factors in IBS patients. Also this Irritable bowel syndrome causing major impact over social behaviour.

Govind K Makharia, Anil K Verma., Prevalence of Irritable Bowel Syndrome: A Community Based Study From Northern India
Fukudo S, Nomura T, Muranaka M, Taguchi F. Brain-gut response to stress and cholinergic stimulation in irritable bowel syndrome. A preliminary study. J Clin Gastroenterol. 1993;17:133–41.
Drossman DA. Presidential address: gastrointestinal ill-ness and biopsychosocial model. Psychosom Med 1998;60: 258±267. Fukudo S, Suzuki J. Colonic motility, autonomic function, and gastrointestinal hormones under psychological stress on irritable bowel syndrome. Tohoku J Exp Med. 1987;151:373–86.