Hypothesis / aims of study
Urinary tract infections (UTIs) are considered as serious global health conundrum, the primary trigger to UTIs is pathogenic bacteria such as Escheichia Coli, Klebsiella pneumonia and staphylococcus saprophyticus. Currently, there is an overwhelming reliance on antibiotics that continuously lead to significant rise in antibiotic resistance. Thus, there must be novel alternative antimicrobial agents with clear-cut efficacy proof. This study aimed to set a comparative analysis and evaluation of Antimicrobial actions of several oils cultivated by organic and conventional farming practices against pathogenic strains of bacteria attacking Urinary Tract Systems (Uropathogenic Bacteria).
The selected oils based on physicochemical properties were Mustard oil, Coconut oil, Sunflower oil, Sesame oil, Garlic oil and Onion Oil.
Study design, materials and methods
The methodology emplemented was the broth microdilution assay; a standardized technique for determining the minimum inhibitory concentration MIC and minimum bacterial concentration MBC of the oils against the selected bacterial strains.
Uropathogenic bacterial suspensions were prepared with a standard concentration and some specific dilution of the oils were exposed to the strains, in order to test and record their inhibition action and neutral bactericidal effects. Controls included positive control with specific antibiotics and negative controls using solvents, were also tested to maximize the validity and reliability of the results.
Interpretation of results
These findings highly recommend the potential of some organic farming oils as profoundly effective Antimicrobial agents to fight against Uropathogenic bacterial and UTI management.